SimGlycan® Workflow

High Throughput Glycopeptide Analysis

Note: Presentation, and Sample Data are Mass spec vendor specific. To download, please click on the appropriate vendor icon first

SimGlycan supports identification of glycopeptides of complex mixtures separated by LC and detected by MS/MS in batch processing mode. The Protein ID, Protein sequence or Peptide sequences identified by using a third party tool are used by SimGlycan as initial input. The software identifies probable glycan-peptide combinations and ranks them on the basis of observed peaks in the MS/MS data corresponding to diagnostic ions from theoretical glycopeptide. High resolution accurate mass data with an error tolerance between 1 to 200 parts per million (ppm) and 0.1 to 2000 milli Dalton (mDa) can be analyzed.

Key Features in the workflow:

  • Supports direct loading of native data files of all major mass spectrometry vendors
  • LC-MS peak detection and picking
  • Support for multiple protein modifications
  • MS/MS annotation
  • Portable report with glycopeptide structure

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