We acknowledge all the authors of the references mentioned below whose publication have been helpful during the development of this program.
Freier, S.M., Kierzek, R., Jaeger, J.A., Sugimoto, N., Caruthers, M.H., Neilson T. and Turner, D.H. (1986) "Improved free-energy parameters for predictions of RNA duplex stability", Proc. Natl. Acad. Sci. USA 83, 9373-9377.
Breslauer, K.J., Frank, R., Blocker, H., and Markey, L. A. (1986) "Predicting DNA duplex stability from the base sequence", Proc. Natl. Acad. Sci. USA 83, 3746-3750.
Handbook of Biochemistry and Molecular Biology (1975) Fasman G.D., ed., 3rd edition, Nucleic Acids - Vol. 1, 589, CRC Press, Cleveland, OH.
Rychlik, W., Spencer, W.J. and Rhoads, R.E. (1990) "Optimization of the annealing temperature for DNA amplification in vitro", Nucleic Acids Res. 18, 6409-6412.