Appendix A: Tables

Net Primer uses various standard values in its formulas. Many of them are given below in tabular form.

Table of Extinction Coefficient values³ (in A₂₆₀ units/µmol):

Pair values -

Second Nucleotide ® First Nucleotide ¯	dA	dC	dG	dT
dA	13.7	10.6	12.5	11.4
dC	10.6	7.3	9.0	7.6
dG	12.6	8.8	10.8	10.0
dT	11.7	8.1	9.5	8.4

Individual values -

dA = 15.4 dT = 8.7 dC = 7.4 dG = 11.5

Table of entropy values of a nearest neighbor nucleotide² (in -cal/°K/mol):

Second Nucleotide ® First Nucleotide ¯	dA	dC	dG	dT
dA	24.0	17.3	20.8	23.9
dC	12.9	26.6	27.8	20.8
dG	13.5	26.7	26.6	17.3
dT	16.9	13.5	12.9	24.0

Table of enthalpy values of a nearest neighbor nucleotide² (in -cal/mol):

Second Nucleotide ® First Nucleotide ¯	dA	dC	dG	dT
dA	9100	6500	7800	8600
dC	5800	11000	11900	7800
dG	5600	11100	11000	6500
dT	6000	5600	5800	9100