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High Throughput Analysis of N-glycans using MS1 Spectra

GlycanExplorer supports analysis of N-glycans using MS1 spectra for both derivatized and native (free reducing end) glycan samples. The supported derivatization methods are Permethylation and fluorophore labeled reducing end modification. As a prerequisite, the users have to create a custom database containing curated N-glycan structures, save experimental information (such as instrument details, ionization technique, derivatization, reducing end fluorophore), and store structure specific MS1 spectra.

The software accepts the experimental precursor mass, ion species, reducing end fluorophore information as input and reports the candidate glycan structures using exact mass search within the user specified mass tolerance. GlycanExplorer identifies the probable glycan structures for multiple adduct combinations in a single search run, saving the time spent on repeated analysis. A glycan with the lowest delta mass is scored the highest and is assigned to the compound of interest.

Once the database search is complete, the software automatically generates a mass spectrum annotating each isotopologues of the identified LC-compound. A portable Microsoft Excel report can also be generated containing the identified glycan structures.


Key Features:

1. Efficient LC-MS Peak Detection and Picking.

2. Detailed database search parameters and filters.

3. Perform database search with multiple adduct combinations in one go.

4. Display MS1 spectrum with annotated isotopologues of the LC compound.

5. Portable Microsoft Excel report generation.

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Resources

Posters

Introducing a Novel Software Containing an Experimental High-Resolution Mass Spectral Library of N-Glycans...

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Blogs

Spectral Matching Vs. in-silico Fragment Matching – Rendering Confidence to Bio-therapeutic Glycan...

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Webinar

GlycanExplorerTM Software for High Throughput Biotherapeutic Glycan Characterization...

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