"The SYBR Green primers designed using Beacon Designer worked extremely well. Pretty good amplification factor of over 90% was observed with no primer-dimers." -Mr. Adelumola Oladeinde, University of Georgia, United States.
"I used Beacon Designer to design primers for several targets. All the designed primers had an efficiency of over 90% and they worked well for me. Excellent R^2 and melt curves were obtained for these primers." -Mr. Islam El-Awaad, Institut für Pharmazeutische Biologie, Technische Universität Braunschweig, Germany.
"Beacon Designer makes designing complex scorpions probes easier. The level of detail included in the primer/probe result is exceptional. I highly recommend this software for designing molecular based assays." -Mr. Justin Ellem, ICPMR Westmead Hospital, Australia.
"I successfully designed different sets of Taqman probes and primers for my genes of interest using Beacon Designer. The multiplex capability of the software was especially useful for designing my assays." -Ms. Sandra Meryl Rodrigues, North Carolina State University, US.
"Beacon Designer is a very good software! I used it for designing not only TaqMan primers and probe but also FRET assays and it fits perfect for my research requirements." -Dr. Matteo Bernabini, University of Pisa, Italy.
"Beacon Designer is a brilliant software. I enjoyed using its user intuitive features which makes it the most popular software amongst researchers." -Ms. Chelsea Back, Murdoch University, Australia.
"Using Beacon Designer, we designed qPCR primers which performed extremely well in our experiments. I found the program both powerful and user-friendly." -Mr. José Nuno Leitao, Institute for Molecular and Cell Biology, IBMC, Portugal.
"Beacon Designer is very easy to use and worked really well for the designing of reverse-transcription quantitative PCR protocol. I am satisfied with the product and it addressed my research needs well." -Ms. Eleonora Sittig, Turku Center for Biotechnology, Finland.
"I used Beacon Designer and found it quite easy to use. It has an excellent interface that gives detailed information on primer properties. It is also very convenient to check primer specificity using the software." -Dr. Wenxiang Fu, NYU Langone Medical Center, US.
"I am VERY impressed with Beacon Designer. I have tested the program to my satisfaction. The program saves a lot of time going to other web sites to evaluate secondary structure of primers, etc. Thanks for a great product!" -Mary L. Taub, State University of New York.
"I really liked PREMIER Biosoft's Beacon Designer. It was very easy to use and provided me with excellent primers."
-Dr. Mohammed Ahmed, University of Bradford, United Kingdom.
"I used Beacon Designer for TaqMan assay design as well as designing probes from previously designed primers. I was able to navigate the program fairly easily so I would give high marks for ease of use. I also very much liked the interface with BLAST for potential regions of cross homology. Overall the program seemed to do a good job of providing template specific primers and probes."
-Dr. Ryan Troyer, Colorado State University, United States.
"Beacon Designer is a very powerful software for designing qRT-PCR and HMRA primers." -Dr. Yi Wang, Agriculture and Agri-Food, Canada.
"I designed SYBR Green primers for 5 different genes using Beacon Designer. They all worked well and I am extremely pleased with the results."
-Dr. Yulia Koryakina, University of Virginia, US.
"Beacon Designer is a great software, all the qPCR primers I designed using it worked perfectly."
-Dr. Maruotti Julien, Johns Hopkins University School of Medicine, USA.
"I enjoyed Beacon Designer very much. I found it more robust, versatile and complete than others I've tried. I was able to design my Taqman probes and primers very easily." -Dr. Carlos Sao-Jose, University of Lisbon, Portugal.
"I am very satisfied with Beacon Designer, I use it in my work. I can design various probes and specific primers, it is very easy for me."
-Dr. Dufková, Veterinary Research Institute, Czech Republic.
"I tried Beacon Designer and it was fantastic. I think this is the most complete qPCR primer design program." -Ms. Andrea Aguado, Universidad Autonoma de Madrid, Spain.
"I was very pleased with Beacon Designer. It was easy and straightforward to use. The primers I ordered based on this software worked well."
-Ms. Allison Light, University of Rochester, USA.
"Beacon Designer works fantastically and very good software for RT PCR primer design." -Mr. Mahmood, Copenhagen University, Denmark.
"Beacon Designer is very easy to use and always gives good primers."
-Dr. Chenggang Wu, The University of Texas Health Science Center at Houston, US.
"I used Beacon Designer to design primers for qPCR. The program was very simple to use and the designed primers worked well."
-Dr. Tina Summerfield, University of Otago, New Zealand.
"Beacon Designer software is an expert in qPCR primer design. All of the 16 primer pairs are excellent and worked well."
-Dr. Hongning Wu, Shantou University, China.
"I designed several primer sets with Beacon Designer™ and have evaluated a few of them. All of them have worked on the first try. This software is quite user-friendly and convenient too. I honestly can't think of any improvements to suggest."
-Dr. Laurie Earls, Developmental Neurobiology, St. Jude Children's Research Hospital.
"I appreciate the intuitive, user friendly interface of Beacon Designer™ as compared to that of primer express, fastPCR and Vector NTI."
-Dr. Hannes Richter,
University of Lausanne (UNIL),
"I became interested in Beacon Designer™ because of some articles I had
read in trade literature.
Once I ran through the tutorial-kudos to the team who designed it--one of the best I've seen for software of this type. I then was able to design 8 sets of primers using the software. I found the software user friendly, and if I got stuck I just replayed the applicable tutorial section. I contacted customer support, and the service far exceeded my expectations. The reason we chose PREMIER Biosoft was because it was highly recommended to us as a highly service oriented company and now after talking to the support, I've seen why that was said."
-Mr. Richard Hanes, Molecular Biology Core Lab Manager, Bowles Center, UNC-Chapel Hill Medical School.
"I used Beacon Designer™ to design oligos for qPCR. Using the program I could control the parameters myself and thus have an "autonomy" over the design. The program automatically avoids the complementary structures during the design, there by enhancing the success rate. I think the software is great and worth the price." -Mathieu Tremblay, Research Assistant, Agriculture and Agrifood Canada.
We are very satisfied with the software and the LNA probes we designed using Beacon Designer™ are working wonderfully."
-Dr. Steven M. Short, Assistant Professor, Department of Biology, University of Toronto Mississauga.
"I used Beacon Designer™ for designing new assays. I found it simple to use and it met my expectations well. The performance was great and I obtained good results. The design options were exhaustive, saving my time." -Meritxell Espino Guarch, IDIBELL-CGMM, Spain.
"I found that designing primers using Beacon Designer™ software has saved me many hours of analyzing potential primers. The program is very user friendly and the layout of the reports allowed all relevant information to be located easily."
-Ms. Stacey Lynch, Department of Veterinary Science,
Melbourne University, Australia.
"I have used Beacon Designer™ to design several different SYBR® Green primers, and they worked out very well. I am impressed with the product." -Dr. Par Towb, Department of Biological Sciences, UCSD, La Jolla, CA, United States.
have found Beacon Designer™ a robust and
sound program which might be really
helpful in designing molecular beacons
and other oligos for real-time PCR." -Dr. Miro Tel, IMEM, Russia.
"From several software
packages that I have tested Beacon Designer™
is probably the best."
Bendezu, Letterkenny Institute of Technology,
"I have been able
to test 10 different primer-pairs designed
for use with SYBR Green by Beacon Designer™
4. They all work very well and I am
very happy with the results!" -Mr.
Benjamin Edvinsson, Karolinska University,
is easy to learn and eliminates costly
guesswork" -Jeremy Pierce, Science,
vol. 294, page 2381, (2001).
"I like Beacon
Designer 2, it does both Beacons and
TaqMan® probes and is pretty intuitive." -Justin T Brown Phd, Cenetron Diagnostics,
"I have used Beacon
Designer on multiple sequences and have
found that the program designs optimal
molecular beacons and primer pairs.
The molecular beacons and primer pairs
it designed produced excellent results
in real-time PCR experiments on the
Bio-Rad's iCycler iQ Detection System." -Larissa Tan, Bio-Rad® Laboratories,
"...fast and accurate ...tool
for the design of molecular beacons
and primer pairs" -The Scientist Full
"To me, the best program to design
TaqMan® probes is Beacon Designer™. I'm
very happy to use it because it is fast
and easy and the probes and primers
I use are working really fine." -Milena Pariali, Italy.
"We are using Beacon Designer™
2 and love it!" -Daniel Inderbitzin.
"I have forwarded design requests
from US customer to Paris, and those
customers have responded very positively
about the success of their experiments" -Dr. Alex Amiet, Applications Manager,
Genomic Tools, Proligo, LLC USA.
"Word of mouth has it that the
Beacon Designer™ software our group uses
gives the best results. All the groups
are now flocking to our lab to use our
copy. What a nuisance! I wish they would
spring for their own copy" -Shelley
Zhang, Univ. of Louisville, Baxter Bl
"The program is easy to use and
I enjoyed using it...Thank you very
much for your very good product" -Dr. Irina Bogdarina.
"Beacon Designer™ 2 was incredibly
straight forward to use. The product
worked great and the beacon I designed
appears to work perfectly" -Freddie
Peyerl, Beth Israel Deaconess Medical
"The program works well. We have
designed primers from this software
FAM-TAMRA labeled and they work well.
this software could state the optimal
temp for pcr reactions" -Anthony
"We love your program - it has
saved time in that we don't have to
use 3 or 4 different programs to determine
how well the probes we have designed
will bind to other sequences. And it
has saved money because I don't have
to purchase several expensive labeled
probes to test which one will work the
best. With Beacon Designer™ 2, we can
design specific probes efficiently and
with the multiplex function save even
more time and feel confident about the
results. Certainly, Beacon Designer™
2 will be able to design effective probes
for detection of single base pair differences." -Dr. Gale Newman, Dept. Micro, Biochem,
Immuno Morehouse School of Medicine,
"The easy to use interface of
Beacon Designer™ saved me a lot of time.
I especially like the ability to design
alternative assays and be able to pick
the one that fits my needs the best" -Dr. Benjamin Edvinsson, Department of
Laboratory Medicine, Karolinska Institute,
"I found the program was extremely
user friendly and fast. I am very happy
with the quality of the results, much
better than any other program that I
tested" -Dr. Ourania Kosti, Department
of Molecular Endocrinology, Division
of General and Developmental Medicine,
Barts and the London, Queen Mary School
of Medicine and Dentistry, London, UK.
"Our success rate has been over
90% when we used the SYBR® Green primers
and TaqMan® probes designed with Beacon
Designer." -Dr. Michael Sheldon, Baylor
College of Medicine, Houston, Texas.
"We have designed several primer/probe
sets for reverse-transcription quantitative
PCR using TaqMan. All have worked very
well. We particularly like the way that
the primers/probes are 'scored'
as best, good or poor, it is much more
convenient than other programs we have
used. We also like the integrated BLAST
feature. We are very satisfied customers
and have recommended the product to
our colleagues and collaborators." -Ms. Laura O'Hara, University of Cambridge,