Designing optimal multiplex primer probe sets that work well and accurately quantitate can be a challenging task. Beacon Designer™ uses innovative proprietary algorithms to design optimal primer-probe sets for single tube multiplex assays using TaqMan® for up to 5 targets. All sets are analyzed for cross compatibility and cross homology to other targets to ensure specific and efficient amplification. If you want to incorporate well-proven sets for some of the templates, or include reference or housekeeping genes for normalization purposes in the assay, Beacon Designer™ is equipped to fully support your needs.
Beacon Designer™ automates the design of real time primers and probes. It is used by molecular biologists worldwide to design successful real time PCR assays. It saves the time and the money involved in failed experiments. Beacon Designer™ is a flexible solution to your real time primer and probe design needs and pays for itself many times over.
You can BLAST search sequences and search for template structures from within the program. The results of both these searches are used while designing primers and probes. The regions that exhibit significant cross homologies and template structures are avoided automatically during the design.