Design TaqMan® Probes
The TaqMan® assay design module in Beacon Designer™ can design TaqMan® probes suitable for single sequence as well as multiplex experiments. Beacon Designer™ supports multiplexing for up to four sequences, with the ability to lock an assay for a reference gene. The primers and probes designed for other sequences in the multiplex set will take into account the Tm and cross dimer values for this assay as a reference.
TaqMan® probes designed by Beacon Designer™ are free from runs, repeats and dimers and are used for SNP genotyping and gene expression profiling.
LNA™ substituted TaqMan® probes are designed by Beacon Designer™ which are stable at higher temperature due to the modified LNA™ bases.
Beacon Designer™ outputs many TaqMan® probe-primer sets for the user to choose from.
TaqMan® assays are ranked according to the proprietary ranking algorithm that weighs many factors considered important for primer and probe design.
Read about how TaqMan® probes work!
Beacon Designer™ automates the design of real time primers and probes. It is used by molecular biologists worldwide to design successful real time PCR assays. It saves the time and the money involved in failed experiments. Beacon Designer™ is a flexible solution to your real time primer and probe design needs and pays for itself many times over.
You can BLAST search sequences and search for template structures from within the program. The results of both these searches are used while designing primers and probes. The regions that exhibit significant cross homologies and template structures are avoided automatically during the design.