December 15, 2020
Quantitation of lipids is significant because lipids have a widespread biological role in the body in terms of intracellular signalling or local hormonal regulation etc. Due to their structural complexity, attaining absolute quantitation of lipids is not feasible experimentally using mass spectrometry. As an alternative, relative quantitation of lipids has been the go-to approach for comprehensive lipidomic analysis. Among the different strategies (like Shotgun or untargeted approach), targeted lipidomics appears as a promising method for comprehensive lipid quantitation. The combination of utilizes Multiple Reaction Monitoring (MRM) transition list and a comprehensive internal standard (IS) strategy makes it real easy to accurately profile and quantify the lipids.
Targeted lipidomics helps to identify and quantitate lipids with both higher sensitivity and specificity. With the use of MRM mode under triple quadrupole mass spectrometry, low abundant lipids can be detected and quantified accurately. However, the bottleneck for this approach has been the data analytics due to the internal standard and quantitation calculations. These challenges are:
SimLipid software offers a comprehensive quantification workflow solving these analytical challenges . To know more, click here.
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