Design Primers for Loop-Mediated Isothermal Amplification

Design Primers for Loop Mediated Isothermal Amplification

LAMP Designer designs efficient primers for Loop-Mediated Isothermal Amplification assays, that amplify DNA and RNA sequences at isothermal conditions, eliminating the necessity of a PCR setup. The technology relies on auto-cycling and DNA polymerase mediated strand displacement DNA synthesis, amplifying a few copies of DNA to 10⁹ in less than an hour. Reverse transcription coupled LAMP can be applied for amplification of RNA sequences.

Avoid Cross Homologies

LAMP Designer automatically interprets BLAST search results and avoids those regions to design primers that have significant cross homologies with the database.

Verification BLAST

The primers can be BLAST searched against a database to verify their specificity.

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