Design Primers for Loop Mediated Isothermal Amplification
LAMP Designer designs efficient primers for Loop-Mediated Isothermal Amplification assays, that amplify DNA and RNA sequences at isothermal conditions, eliminating the necessity of a PCR setup. The technology relies on auto-cycling and DNA polymerase mediated strand displacement DNA synthesis, amplifying a few copies of DNA to 109 in less than an hour. Reverse transcription coupled LAMP can be applied for amplification of RNA sequences.
LAMP employs four specially designed primers (two inner and two outer) that recognize six distinct regions in the target DNA. Hybridization of the four primers to the target DNA is a very crucial step for the efficiency of LAMP. The design of these four primers is therefore critical for a successful assay.
Avoid Cross Homologies
LAMP Designer automatically interprets BLAST search results and avoids those regions to design primers that have significant cross homologies with the database.
The primers can be BLAST searched against a database to verify their specificity.
Multiplex LAMP Primer Set
The primers designed for a sequence can be checked for multiplexing. The free energies of the most stable cross homologies between the primers designed, is displayed by the program.
The designed primer sets, along with their properties, can be exported in standard csv or excel formats.
Data & Database Management
Multiple projects can be created. Data of multiple experiments can be easily managed by creating a separate project for each experiment. It maintains a local database for sequence information and search results.