| Sl. # |
Method |
Separation Method |
MS Instrument |
MS/MS Data Acquisition |
Literature |
| 1 |
Normal Phase or Reverse Phase LC-QTOF MS |
- RP separations: Agilent Zorbax EclipsePlus RRHD, 2.1 x 100mm, 1.8 µm column with a binary gradient
- NP separations: Agilent Zorbax Rx-Sil, 2.1 x 100mm, 1.8 µm column with a binary gradient on an Agilent 1290 UHPLC system
|
Agilent 6540 Q-TOF with Dual Agilent Jet Stream ESI Source |
DDA |
Authors: Meitei, N.S., Biswas, A., Apte, A., Madden, S., Sartain, M.
Title: Automatic lipid characterization using SimLipid® from normal phase and reverse phase liquid chromatography -MS, MS/MS data acquired in variable ion modes
Conference: 63rd ASMS Conference on Mass Spectrometry & Allied Topics (2015), St. Louis
Link |
| 2 |
SFC -QTOF MS |
Columns 1, and 2 connected in series:
- EC-C18
-
hybrid UPC2 BEH
|
Agilent 6540 Q-TOF MS |
DDA |
Authors: Mohsin, S.B., Williams, K., Renu, D., Meitei, N.S., Kalakoti, S., Madden, S., Kitagawa, N.
Title: Development of an Integrated Workflow for Profiling and Semi-Quantitative Analysis of Lipids
Conference: 65th ASMS Conference on Mass Spectrometry and Allied Topics (2017), Indianapolis, IN
Link |
| 3 |
LC-QTOF MS |
HPLC Instrumentation:
Agilent 1290 Infinity
Column – ACQUITY UPLC CSH C18 2.1 x 100 mm, 1.7 μm
|
Bruker Impact II UHR-QqTOF MS |
DDA |
Authors: Meitei, N.S., Gupta, H., Apte, A., Barsch, A., Alving, A., Henao, J.J.A., Stark, K.D.
Title: Automated Lipid Profiling of a Plasma Sample Using Ultra-High Resolution Qq-Time-Of-Flight Impact II™ Mass Spectrometer with SimLipid Software
Conference: 65th ASMS Conference on Mass Spectrometry and Allied Topics (2017), Indianapolis, IN
Link |
| 4 |
MALDI-FTICR |
N/A |
Bruker 15T SolariX FTICR-MS |
N/A |
Boughton, B. and Roessner, U. (2015). Imaging Mass Spectrometry for Spatial Metabolomics. Aus. Biochemist, 46(3): 9-11.
Link |
| 5 |
Waters®
UPLC- QTOF(SONAR) MS |
CSH 1.7 μm C18 reversed phase (RP) 2.1 x 100 mm LC column
|
Waters Xevo G2-XS QtoF (
operated in
SONARTM mode) MS
|
DIA |
Authors: Gethings, L. A., Meitei, N.S., Vissers, J.P.C., Heywood, D., Castro-Perez, J., Langridge, J. I.,
Title: Utilization Of SimLipid for the Characterization of Metabolic Syndrome Related Lipids Acquired Uing a Novel
Scanning Quadrupole DIA Acquisition Method.
Conferences: 13th Annual Conference of the Metabolomic Society (2017), Brisbane, Australia & 65th ASMS Conference on Mass Spectrometry and Allied Topics (2017), Indianapolis, IN
Link |
| 6 |
Waters®
UPLC-TOF(
HDMSE) MS |
LC system:
ACQUITY UPLC
Column:
ACQUITY UPLC CSH C18
2.1 x 100 mm, 1.7 μm
|
Waters SYNAPT G2 HDMS System
|
DIA |
Authors: Isaac, G., McDonald, S., Astarita, G.
Title: Automated Lipid Identification Using UPLC/HDMSE in Combination with SimLipid
Waters Application Note No. 720004169EN
Link |
| 7 |
Waters®
UPLC- TOF (MSE) MS |
LC system:
ACQUITY UPLC
with 2D Technology
Column:
1st Dimension Column: UPLC BEH HILIC 2.1 x 100mm, 1.7 μm
2nd Dimension Column: UPLC CSH C18 2.1 x 100mm, 1.7 μm
Trap Column: UPLC VanGuard CSH C18 2.1 x 5mm |
Waters SYNAPT G2 HDMS System |
DIA |
Authors: Netto, J.D., Sing, S.F., Ditcham, W., Borowitzka, M., Ritchie, M.
Title: Inter-Strain Comparative Lipidomics of Microalgae Using Multi-Dimensional Separation Techniques
Conference: 19th International Mass Spectrometry Conference (IMSC), 2012, Kyoto.
Link |
| 9 |
LC-Orbitrap based MS |
Accela 1250 quaternary pump with Accela Open autosampler |
Thermo Fisher Scientific LTQ Orbitrap Velos Pro Hybrid MS |
DDA |
Authors: Meitei, N.S., Arun Apte, Rahlouni, F., Sullivan, D. J., Shulaev, V.
Title: Automated Lipid Profiling of Malaria Samples Using Orbitrap Velos Pro Mass Spectrometer with LipidSearch and SimLipid Software
Conference: 65th ASMS Conference on Mass Spectrometry and Allied Topics (2017), Indianapolis, IN
Link |
| 10 |
HPLC- TOF MS |
HPLC: Acquity HSS T3 1.8μm, 2.1 x 100mm @ 60oC |
SCIEX TripleTOF® 5600 System |
DDA |
Authors: Meitei, N.S., Sreenidhi, A.K., Apte, A., & Ubhi, B.K.
Title: LC-MS and MS/MS based Large Metabolomic Data Processing and Analysis using SimMet®.
Conference: 63rd ASMS Conference on Mass Spectrometry & Allied Topics (2015), St. Louis.
Link |
| 11 |
MALDI-MS/MS |
N/A |
SCIEX TOF/TOF™ 5800 system |
DDA |
Authors: Meitei, N.S., Apte, A., Waidelich, D., Abdi, F., Glueckmann, M.
Title: Automatic Characterization of Lipids Using Charge Remote Fragmentation Ions and Peaks Characteristic of Fatty Acid Fragmentation From MALDI MS/MS Data
Conference: 62nd ASMS Conference on Mass Spectrometry and Allied Topics (2014), Baltimore
Link |
| 12 |
Shotgun based Precursor Ion/Neutral Loss Scanning experiments
|
1. N/A
2. HPLC: Phenomenex Kinetex C8 (150 × 2.1 mm, 2.6 μm) @ 45o C |
1. Shimadzu LCMS-8040
2. Shimadzu LCMS-8060
|
|
1.
Authors: Gupta, R., Sadowski, M., Meitei, N. S., Blanksby, S. J.
Title: Shotgun Lipidomics of Prostate Cancer Cells Using ESI-MS Shimadzu 8050 and Simplified Data Analysis by SimLipid Software
Conference: 13th Annual Conference of the Metabolomic Society (2017), Brisbane, Australia
Link 1
2.
Authors: Nakanishi, T.
Title: Phospholipid analysis using SimLipid software
Shimadzu Document No. LAAN-A-LM-E120
Link 2 |
