NASBA® Assay Design
NASBA® assays are gaining acceptance among the molecular biology researchers as genetic material identification technique. NASBA® stands for Nucleic Acid Sequence Based Amplification and is an isothermal amplification process. NASBA® assays detect the mRNA in double stranded DNA without needing temperature cycling.
Beacon Designer™ can design molecular beacons for NASBA® assays for single template, multiplex or allelic discrimination. You can also evaluate pre-designed primers and probes for NASBA® assays. While evaluating primers, Beacon Designer™ automatically adds a purine tag to P1 primer sequence when necessary.