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NASBA® Assay Design

NASBA® assays are gaining acceptance among the molecular biology researchers as genetic material identification technique. NASBA® stands for Nucleic Acid Sequence Based Amplification and is an isothermal amplification process. NASBA® assays detect the mRNA in double stranded DNA without needing temperature cycling.

Beacon Designer™ can design molecular beacons for NASBA® assays for single template, multiplex or allelic discrimination. You can also evaluate pre-designed primers and probes for NASBA® assays. While evaluating primers, Beacon Designer™ automatically adds a purine tag to P1 primer sequence when necessary.

Beacon Designer software designs molecular beacons for NASBA assays. The software also evaluates pre-designed primers and probres for NASBA assays.

Automate Your Real Time PCR Primer and Probe Design

Beacon Designer™ automates the design of real time primers and probes. It is used by molecular biologists worldwide to design successful real time PCR assays. It saves the time and the money involved in failed experiments. Beacon Designer™ is a flexible solution to your real time primer and probe design needs and pays for itself many times over.

Specific and Efficient Design: How Beacon Designer™ Makes it Possible?

You can BLAST search sequences and search for template structures from within the program. The results of both these searches are used while designing primers and probes. The regions that exhibit significant cross homologies and template structures are avoided automatically during the design.


  • SYBR® Green Primer Design
  • TaqMan® Probe Design
  • Multiplex Real Time PCR Assay Design
  • HRMA Primer Design
  • MethyLight TaqMan® Design
  • Molecular Beacon Design
  • NASBA® Assay Design
  • FRET Probe Design
  • Scorpions® Design
  • Avoiding Cross Homology- For Specific Oligo Design
  • Avoid Template Structures- Increase Primer Efficiency
  • Oligo Rating


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