Molecular Beacon Design
Beacon Designer™ designs optimal molecular beacons for routine qPCR and NASBA® assays. The program automatically selects a stem of appropriate length (5, 6 or 7 bp long) for optimal beacon Tm. These beacons are not only have a suitable Tm, but are also free from runs, repeats and dimers for maximum signal fidelity. The program displays the graphical structure of the beacon along with its secondary structures.
Read the tech note on molecular beacons.