Net Primer
Free! Primer Analysis Software
"I wish I had analyzed my primers before ordering for the third time. Now I check all my primers with NetPrimer before ordering" -Stacey McCann, Cancer Biology Program, Stanford University
About NetPrimer
NetPrimer combines the latest primer analysis algorithms with a web-based interface allowing the user to analyze primers over the Internet. All primers are analyzed for primer melting temperature using the nearest neighbor thermodynamic theory to ensure accurate Tm prediction. Primers are analyzed for all primer secondary structures including hairpins, self-dimers, and cross-dimers in primer pairs. This ensures the availability of the primer for the reaction as well as minimizing the formation of primer dimer. The program eases quantitation of primers by calculating primer molecular weight and optical activity. To facilitate the selection of an optimal primer, each primer is given a rating based on the stability of its secondary structures. A comprehensive analysis report can be printed for individual primers or primer pairs.
Major Features
- Primer Melting Temperature (Tm): Predicts primer melting temperature using the accurate nearest neighbor thermodynamic
algorithm.
- Primer Properties: Tm , molecular
weight, GC%, optical activity
(both in nmol/A260 & µg/A260),
DG, 3' end stability, DH, DS,
and 5' end DG.
- Primer Secondary Structures: Hairpins, dimers, cross dimers, palindromes,
repeats and runs.
- Primer Rating: Quantitative prediction of the efficiency of a primer.
- Comprehensive Report: Prints complete primer analysis for an individual
primer or primer pair.
- Primer Pairs: Analyze individual primers or primer pairs.
- Comprehensive Help: Details
all the formulas and references used in primer
analysis algorithm.
