High Throughput Glycopeptide Analysis

SimGlycan supports identification of glycopeptides of complex mixtures separated by LC and detected by MS/MS in batch processing mode. The Protein ID, Protein sequence or Peptide sequences identified by using a third party tool are used by SimGlycan as initial input. The software identifies probable glycan-peptide combinations and ranks them on the basis of observed peaks in the MS/MS data corresponding to diagnostic ions from theoretical glycopeptide. High resolution accurate mass data with an error tolerance between 1 to 200 parts per million (ppm) and 0.1 to 2000 milli Dalton (mDa) can be analyzed.

Key Features in the workflow:

• Supports direct loading of native data files of all major mass spectrometry vendors
• LC-MS peak detection and picking
• Support for multiple protein modifications
• MS/MS annotation
• Portable report with glycopeptide structure