Design Tiling Arrays using Array Designer
Amplify Whole Genomes with Tiling Primers and Probes Using Array Designer
Array Designer can automate the design of primers and probes for tiling arrays. With Array Designer, you can design tiling arrays to spot every base of a long genomic sequence while avoiding repetitive regions.
Array Designer helps you spot each and every base of the target DNA sequence using the 'Overlapping Amplicon' option. As the name suggests, this option outputs primers that produce overlapping amplicons so that no base pair is left undetected. The other option available for primer design is the 'Distinct Amplicon' option. The amplicons generated could be as close as one base pair apart, depending on the sensitivity you choose.
Primer Design for Tiling Array
- Array Designer helps you redesign tiling primers for the failed fragments by relaxing the design parameters.
Probe Design for Tiling Array
- Array Designer designs overlapping probes tiled across the genome to cover an entire genomic region of interest facilitating genome wide analysis.
Whole Genome Amplification
Genome-wide tiling arrays can overcome many of the limitations of the previous approaches by comprehensively probing transcription in all regions of the genome. Whole genome high density tiling arrays provide a universal platform for genomic analysis through whole genome amplification. Tiling microarrays through whole genome amplification allow you to explore other genome-wide structure/function relationships, including global studies of transcription factor binding sites, sites of chromatin modification, sites of DNA methylation and even chromosomal origins of replication.