High Throughput Primer Design: Use Xpression Primer's sophisticated algorithm to design thousands of primers in a single search run. The program uses statistical optimization techniques to select the best primer pair for each sequence based on your selection criteria.
Extensive Assay Support: Xpression Primer provides comprehensive support to high throughput cloning systems such as Gateway®, BD In-Fusion™, epitope and TOPO® tools. To work with other expression systems, add functional tags of your choice and design tagged primers.
Sophisticated Sequencing Primer Design: Xpression Primer designs optimal primers for multiples sequences in a single search run.
Generate Precise Transcripts: Design primers to generate sense or antisense transcripts for in vitro expression studies.
Web Savvy: Xpression Primer searches Entrez and downloads batches of ORF sequences directly into the program.
Lightning Fast: Processes three hundred 10kb sequences in approximately 2 minutes.
Use the sophisticated algorithm of Xpression Primer to design thousands of tagged primers for expression cloning systems such as Gateway®, BD In-Fusion™, epitope and TOPO® Tools. You can choose to amplify an entire ORF or generate N terminal or C terminal fusion proteins. Xpression Primer ensures that the reading frame of the amplified ORF is conserved. To work with other expression systems, simply add functional tags of your choice and design tagged primers.
To ensure the success of your PCR experiment, let Xpression Primer design nested tagged primers to amplify ORFs. You can locate the outer primers anywhere in the UTRs or in regions of no significant homology. Xpression Primer will BLAST your sequences, automatically interpret the results and design highly specific primers. The tagged inner primer pair amplifies the PCR product generated by the outer pair with little or no non-coding regions. You can also choose from a list of alternate primers to better meet specific experimental needs.