rt-PCR Primer Design
Beacon Designer™ is the only qPCR assay design software that supports design of qPCR primers (including rt-pcr primers) optimized for different probe chemistries. The design criteria for qpcr (and rt-pcr) primers varies slightly between different chemistries but can have a huge impact on the experimentation success rate. Beacon Designer™ designs primers for TaqMan®, molecular beacons, FRET, MethyLight, Scorpions®, LNA™ Substituted TaqMan® and NASBA® assays. Beacon Designer supports rt-pcr oligo design and analysis (evaluation) in following ways:
Beacon Designer™ automates the design of real time primers and probes. It is used by molecular biologists worldwide to design successful real time PCR assays. It saves the time and the money involved in failed experiments. Beacon Designer™ is a flexible solution to your real time primer and probe design needs and pays for itself many times over.
You can BLAST search sequences and search for template structures from within the program. The results of both these searches are used while designing primers and probes. The regions that exhibit significant cross homologies and template structures are avoided automatically during the design.