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Molecular Beacon Design
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Tm Adjustment: Automatically selects a stem of appropriate length for optimal beacon Tm.
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Optimal Beacons: Designs beacons free of dimers, repeats, and runs for increased signal strength.
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Multiplex Beacons & Primers: Checks molecular beacons for cross homologies with all primers preventing competition in multiplex reactions.
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Allele Identification: Designs molecular beacons for detection of both wild and mutant alleles.
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Tm Calculation: Highly accurate hairpin Tm calculation.
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Evaluate Pre-designed Molecular Beacon Assays: Pre-designed primer sets can be evaluated or primers for a pre-designed molecular probe can be constructed. Similarly, a pre-designed beacon can be evaluated or a beacon can be designed for a pre-designed primer set. This facilitates using SYBR® Green primers for beacon assays which can be used for bacterial identification assays or any other assay.
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Lightning Fast: Processes an average cDNA sequence in under a second.
- Graphical View: Displays designed beacon graphically along with its properties.
Read about molecular beacons.
