Design TaqMan® Probes

• TaqMan® Probes: AlleleID® designs optimal TaqMan® probes free of dimers, repeats and runs to ensure signal fidelity.

• TaqMan® MGB Probes: AlleleID® supports the design of TaqMan® MGB probes which are typically shorter as compared to the regular TaqMan® probes.
  

• Multiplex & Allele Identification Assays: AlleleID® supports multiplexing for up to four sequences, avoids cross homologies with all dual labeled probes and primers preventing competition in multiplex reactions. Designs wild and mutant TaqMan® probes for SNP identification.

• Evaluate Pre-designed TaqMan® Probes Based Assays: Pre-designed primer sets can be evaluated and a compatible TaqMan® probe designed. Similarly, a pre-designed TaqMan® probe can be evaluated and compatible primers designed. You can even import the primers designed in the SYBR® Green primer design mode and then design a compatible probe.

• Graphical View: Displays a graphical view of TaqMan® probe secondary structures.

• Ranking: Only the best TaqMan® probes are designed for each template, using statistical optimization technique.

Read about how TaqMan® probes work.