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High throughput glycan & glycopeptide identification tool using mass spectrometry data

SimGlycan predicts structure of glycans from the MS/MS and multi-stage mass spectrometry (MSn) data. Furthermore, comprehensive support for resolving glycopeptides using LC-MS/MS glycopeptide data facilitates glycosylation studies. SimGlycan accepts experimental MS/MS data, matches them with its own database and finally generate a list of ranked candidate structures. Rank of each candidate indicates the proximity between the candidate structure and the experimental glycan. The rank is calculated based on a robust scoring mechanism that considers structure specific diagnostic ions observed in the experimental MS/MS spectra as well as the intensity of those observed peaks.

SimGlycan also supports multistage mass spectrometry (MSn) data analysis for isomers differentiation.


Posters and Presentations

Automated and Rapid Characterization of Low-Molecular-Weight Heparin Oligosaccharides Using TripleTOF 5600 Mass Spectrometer...

SimGlycan: Informatics Support for Automated Characterization of Low-Molecular-Weight...

Application Notes

A Unique Workflow for Glycoprotein Characterization from Sample Preparation to MS/MS Spectral Interpretation

Albanese, J., Lee, R. (2011)
J Biomol Tech., 22(Suppl), S57

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