Species
Identification Assays/Cross Species Assays/Allele
Identification Assays
AlleleID® aligns sequences
using ClustalW and
analyzes conserved and species specific regions. You can then use the
program for real
time PCR primer design (SYBR® Green primer design included) and dual labeled probe design (TaqMan® probes, TaqMan® MGB probes and molecular beacons). These assays are designed to detect only the
strain (strain detection) or species of interest from the mix.
Sophisticated Algorithms for Assay Success
Highly specific oligos are designed by avoiding regions of significant homologies found by automatically interpreting BLAST search results. Real time PCR primer & probe efficiency is enhanced by avoiding template secondary structures. "Minimal Set", one of the
most innovative features in the program, helps design the fewest
number of allele specific oligonucleotide primers and dual labeled probes that
uniquely identify each of the desired species/strain/taxa from the
mix, lowering assay costs. For taxa or cross species assays, this feature is especially useful when the group or taxa is
highly dissimilar. For a partial set of pre-designed, proven set of
primers, AlleleID® can design compatible primers and probes for the
rest of sequences for species identification or taxa specific
assays.
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